{"help":"https://catalogue.cioos.ca/api/3/action/help_show?name=package_show","success":true,"result":{"id":"c7a168e8-f15e-446f-a49d-0b4a1f043ac2","ecv":[],"eov":["subSurfaceSalinity","subSurfaceTemperature","fishAbundanceAndDistribution"],"url":null,"name":"ca-cioos_b4844ae2-d9c1-42f2-ae4d-a8f994c395a0","tags":[],"type":"dataset","notes":"Les fjords sont des estuaires en eau profonde qui abritent un large éventail d'habitats marins pour les poissons pélagiques. Pourtant, les lacunes dans les connaissances sur la distribution des poissons et l'utilisation de l'habitat limitent la mise en œuvre de stratégies efficaces de conservation marine dans les fjords. En utilisant les fjords de la Colombie-Britannique comme étude de cas, nous avons démontré que le métabarcodage de l'ADNe combiné aux caractéristiques des espèces peut améliorer la détection et la surveillance des espèces prioritaires pour la conservation (CPS) dans le cadre de plans de conservation systématiques. \n\nDes échantillons ont été prélevés à quatre moments : fin janvier/février (hiver), mai (printemps), août (été) et novembre (automne) 2023 dans les cinq fjords de la Colombie-Britannique : Indian Arm (səl ̓ilwət in hən̓q̓əmin̓əmde), Howe Sound (Átl'a7tsem à Squamish), Toba Inlet (yṣkamṣamn à Homalco), Bute Inlet et Rivers Inlet (Wánu-vaxsiwa à Oowekyala). Trois à cinq stations ont été échantillonnées dans chaque fjord et des échantillons discrets d'ADN environnemental (ADNe) de 2 L ont été prélevés à l'aide de bouteilles Niskin à la surface, à 10 m sous la surface, à 100 m sous la surface et à 10 m au-dessus du fond. Les données de température et de salinité ont également été collectées à chaque instant à l'aide d'un CTD.\n\nÀ partir des échantillons d'ADNe, la communauté de poissons a été cartographiée à l'aide d'un métabarcoding ciblant une petite région du gène de l'ARNr 12S mitochondrial à l'aide du jeu d'amorces universelles miFISH-U. Des bibliothèques de séquençage indépendantes ont été préparées pour chacun des quatre ensembles de données saisonniers, et chacun a été séquencé à l'aide de la chimie Illumina MiSeq V3 au Hakai Genome Lab sur l'île Quadra, en Colombie-Britannique.\n\nLes profils d'ADNe spatiotemporels ont confirmé la présence d'eulakane (Thaleichthys pacificus) en voie de disparition dans trois fjords et ont identifié les habitats des fjords profonds comme des priorités de conservation pour les espèces mésopélagiques, notamment les élasmobranches sensibles et les migrateurs verticaux quotidiens tels que la lampfish nordique (Stenobrachius leucopsarus). Les pics d'ADNe de plusieurs CPS en hiver et au printemps correspondaient aux périodes de frai documentées, soulignant l'importance des fjords Les morues comme habitat de frai saisonnier. 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[{\\\"date-parts\\\": [\\\"2026\\\", \\\"05\\\", \\\"17\\\"]}], \\\"abstract\\\": \\\"Fjords are deep-water estuaries that support a wide range of marine habitats for pelagic fish. Yet, knowledge gaps in fish distribution and habitat use limit the implementation of efficient marine conservation strategies within fjords. Using British Columbia fjords as a case study, we demonstrated that eDNA metabarcoding combined with species traits can enhance the detection and monitoring of conservation priority species (CPS) within systematic conservation planning frameworks. \\n\\nSamples were collected at four time-points: late January/February (winter), May (spring), August (summer), and November (fall) 2023 from the five British Columbia Fjords: Indian Arm (s\\u0259l \\u0313ilw\\u0259t in h\\u0259n\\u0313q\\u0313\\u0259min\\u0313\\u0259m\\u0313), Howe Sound (\\u00c1tl'\\u1e35a7tsem in Squamish), Toba Inlet (y\\u025bk\\u02b7am\\u025bn in Homalco), Bute Inlet, and Rivers Inlet (W\\u00e1nu\\u01e7vaxsiwa in Oowekyala). Three to five stations were sampled within each fjord and discrete 2 L environmental DNA (eDNA) samples were collected using Niskin bottles at the surface, 10 m below the surface, 100 m below the surface, and 10 m above the bottom.  \\n\\nFrom the eDNA samples, the fish community was mapped using metabarcoding targeting a small region of the mitochondrial 12S rRNA gene using MiFish-U universal primer set. Independent sequencing libraries were prepared for each of the four seasonal datasets, and each was sequenced using Illumina MiSeq V3 chemistry at the Hakai Genome Lab on Quadra Island, BC.\\n\\nSpatiotemporal eDNA patterns confirmed the presence of endangered eulachon (Thaleichthys pacificus) across three fjords and identified deep fjord habitats as conservation priorities for mesopelagic species, including sensitive elasmobranchs and diel vertical migrators such as northern lampfish (Stenobrachius leucopsarus). eDNA peaks for several CPS during winter and spring matched documented spawning windows, underscoring the importance of fjords as seasonal spawning habitat. This globally transferable approach can supplement local stewardship initiatives and long-term monitoring efforts, contributing to more effective marine conservation area design and management.\\\", \\\"edition-date\\\": \\\"\\\", \\\"publisher\\\": \\\"University of British Columbia\\\", \\\"title\\\": \\\"Putting fjord biodiversity on the map for British Columbia conservation planning\\\", \\\"version\\\": \\\"1.0\\\", \\\"DOI\\\": \\\"10.21966/n141-m959\\\", \\\"language\\\": \\\"en\\\", \\\"URL\\\": \\\"https://catalogue.hakai.org/dataset/ca-cioos_b4844ae2-d9c1-42f2-ae4d-a8f994c395a0?local=en\\\"}]","fr":"[{\\\"type\\\": \\\"dataset\\\", \\\"id\\\": \\\"ca.cioos_b4844ae2-d9c1-42f2-ae4d-a8f994c395a0\\\", \\\"author\\\": [{\\\"given\\\": \\\"Lo\\u00efc\\\", \\\"family\\\": \\\"Jacquemot\\\"}, {\\\"given\\\": \\\"Andreas\\\", \\\"family\\\": \\\"Novotny\\\"}, {\\\"given\\\": \\\"Clare\\\", \\\"family\\\": \\\"Adams\\\"}, {\\\"given\\\": \\\"Rute\\\", \\\"family\\\": \\\"Clemente-Carvalho\\\"}, {\\\"given\\\": \\\"Drew\\\", \\\"family\\\": \\\"Jordison\\\"}, {\\\"given\\\": \\\"Colleen\\\", \\\"family\\\": \\\"Kellogg\\\"}, {\\\"given\\\": \\\"Matthew\\\", \\\"family\\\": \\\"Lemay\\\"}, {\\\"given\\\": \\\"Brian\\\", \\\"family\\\": \\\"Hunt\\\"}], \\\"issued\\\": [{\\\"date-parts\\\": [\\\"2026\\\", \\\"05\\\", \\\"17\\\"]}], \\\"abstract\\\": \\\"Les fjords sont des estuaires en eau profonde qui abritent un large \\u00e9ventail d'habitats marins pour les poissons p\\u00e9lagiques. Pourtant, les lacunes dans les connaissances sur la distribution des poissons et l'utilisation de l'habitat limitent la mise en \\u0153uvre de strat\\u00e9gies efficaces de conservation marine dans les fjords. En utilisant les fjords de la Colombie-Britannique comme \\u00e9tude de cas, nous avons d\\u00e9montr\\u00e9 que le m\\u00e9tabarcodage de l'ADNe combin\\u00e9 aux caract\\u00e9ristiques des esp\\u00e8ces peut am\\u00e9liorer la d\\u00e9tection et la surveillance des esp\\u00e8ces prioritaires pour la conservation (CPS) dans le cadre de plans de conservation syst\\u00e9matiques. \\n\\nDes \\u00e9chantillons ont \\u00e9t\\u00e9 pr\\u00e9lev\\u00e9s \\u00e0 quatre moments\\u00a0: fin janvier/f\\u00e9vrier (hiver), mai (printemps), ao\\u00fbt (\\u00e9t\\u00e9) et novembre (automne) 2023 dans les cinq fjords de la Colombie-Britannique\\u00a0: Indian Arm (s\\u0259l \\u0313ilw\\u0259t in h\\u0259n\\u0313q\\u0313\\u0259min\\u0313\\u0259mde), Howe Sound (\\u00c1tl'a7tsem \\u00e0 Squamish), Toba Inlet (y\\u1e63kam\\u1e63amn \\u00e0 Homalco), Bute Inlet et Rivers Inlet (W\\u00e1nu-vaxsiwa \\u00e0 Oowekyala). Trois \\u00e0 cinq stations ont \\u00e9t\\u00e9 \\u00e9chantillonn\\u00e9es dans chaque fjord et des \\u00e9chantillons discrets d'ADN environnemental (ADNe) de 2 L ont \\u00e9t\\u00e9 pr\\u00e9lev\\u00e9s \\u00e0 l'aide de bouteilles Niskin \\u00e0 la surface, \\u00e0 10 m sous la surface, \\u00e0 100 m sous la surface et \\u00e0 10 m au-dessus du fond. Les donn\\u00e9es de temp\\u00e9rature et de salinit\\u00e9 ont \\u00e9galement \\u00e9t\\u00e9 collect\\u00e9es \\u00e0 chaque instant \\u00e0 l'aide d'un CTD.\\n\\n\\u00c0 partir des \\u00e9chantillons d'ADNe, la communaut\\u00e9 de poissons a \\u00e9t\\u00e9 cartographi\\u00e9e \\u00e0 l'aide d'un m\\u00e9tabarcoding ciblant une petite r\\u00e9gion du g\\u00e8ne de l'ARNr 12S mitochondrial \\u00e0 l'aide du jeu d'amorces universelles miFISH-U. Des biblioth\\u00e8ques de s\\u00e9quen\\u00e7age ind\\u00e9pendantes ont \\u00e9t\\u00e9 pr\\u00e9par\\u00e9es pour chacun des quatre ensembles de donn\\u00e9es saisonniers, et chacun a \\u00e9t\\u00e9 s\\u00e9quenc\\u00e9 \\u00e0 l'aide de la chimie Illumina MiSeq V3 au Hakai Genome Lab sur l'\\u00eele Quadra, en Colombie-Britannique.\\n\\nLes profils d'ADNe spatiotemporels ont confirm\\u00e9 la pr\\u00e9sence d'eulakane (Thaleichthys pacificus) en voie de disparition dans trois fjords et ont identifi\\u00e9 les habitats des fjords profonds comme des priorit\\u00e9s de conservation pour les esp\\u00e8ces m\\u00e9sop\\u00e9lagiques, notamment les \\u00e9lasmobranches sensibles et les migrateurs verticaux quotidiens tels que la lampfish nordique (Stenobrachius leucopsarus). Les pics d'ADNe de plusieurs CPS en hiver et au printemps correspondaient aux p\\u00e9riodes de frai document\\u00e9es, soulignant l'importance des fjords Les morues comme habitat de frai saisonnier. Cette approche transf\\u00e9rable \\u00e0 l'\\u00e9chelle mondiale peut compl\\u00e9ter les initiatives locales d'intendance et les efforts de surveillance \\u00e0 long terme, contribuant ainsi \\u00e0 une conception et \\u00e0 une gestion plus efficaces des aires marines de conservation.\\\", \\\"edition-date\\\": \\\"\\\", \\\"publisher\\\": \\\"University of British Columbia\\\", \\\"title\\\": \\\"Mettre la biodiversit\\u00e9 des fjords sur la carte pour la planification de la conservation en Colombie-Britannique\\\", \\\"version\\\": \\\"1.0\\\", \\\"DOI\\\": \\\"10.21966/n141-m959\\\", \\\"language\\\": \\\"fr\\\", \\\"URL\\\": 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Yet, knowledge gaps in fish distribution and habitat use limit the implementation of efficient marine conservation strategies within fjords. Using British Columbia fjords as a case study, we demonstrated that eDNA metabarcoding combined with species traits can enhance the detection and monitoring of conservation priority species (CPS) within systematic conservation planning frameworks. \n\nSamples were collected at four time-points: late January/February (winter), May (spring), August (summer), and November (fall) 2023 from the five British Columbia Fjords: Indian Arm (səl ̓ilwət in hən̓q̓əmin̓əm̓), Howe Sound (Átl'ḵa7tsem in Squamish), Toba Inlet (yɛkʷamɛn in Homalco), Bute Inlet, and Rivers Inlet (Wánuǧvaxsiwa in Oowekyala). Three to five stations were sampled within each fjord and discrete 2 L environmental DNA (eDNA) samples were collected using Niskin bottles at the surface, 10 m below the surface, 100 m below the surface, and 10 m above the bottom.  \n\nFrom the eDNA samples, the fish community was mapped using metabarcoding targeting a small region of the mitochondrial 12S rRNA gene using MiFish-U universal primer set. Independent sequencing libraries were prepared for each of the four seasonal datasets, and each was sequenced using Illumina MiSeq V3 chemistry at the Hakai Genome Lab on Quadra Island, BC.\n\nSpatiotemporal eDNA patterns confirmed the presence of endangered eulachon (Thaleichthys pacificus) across three fjords and identified deep fjord habitats as conservation priorities for mesopelagic species, including sensitive elasmobranchs and diel vertical migrators such as northern lampfish (Stenobrachius leucopsarus). eDNA peaks for several CPS during winter and spring matched documented spawning windows, underscoring the importance of fjords as seasonal spawning habitat. This globally transferable approach can supplement local stewardship initiatives and long-term monitoring efforts, contributing to more effective marine conservation area design and management.","fr":"Les fjords sont des estuaires en eau profonde qui abritent un large éventail d'habitats marins pour les poissons pélagiques. Pourtant, les lacunes dans les connaissances sur la distribution des poissons et l'utilisation de l'habitat limitent la mise en œuvre de stratégies efficaces de conservation marine dans les fjords. En utilisant les fjords de la Colombie-Britannique comme étude de cas, nous avons démontré que le métabarcodage de l'ADNe combiné aux caractéristiques des espèces peut améliorer la détection et la surveillance des espèces prioritaires pour la conservation (CPS) dans le cadre de plans de conservation systématiques. \n\nDes échantillons ont été prélevés à quatre moments : fin janvier/février (hiver), mai (printemps), août (été) et novembre (automne) 2023 dans les cinq fjords de la Colombie-Britannique : Indian Arm (səl ̓ilwət in hən̓q̓əmin̓əmde), Howe Sound (Átl'a7tsem à Squamish), Toba Inlet (yṣkamṣamn à Homalco), Bute Inlet et Rivers Inlet (Wánu-vaxsiwa à Oowekyala). Trois à cinq stations ont été échantillonnées dans chaque fjord et des échantillons discrets d'ADN environnemental (ADNe) de 2 L ont été prélevés à l'aide de bouteilles Niskin à la surface, à 10 m sous la surface, à 100 m sous la surface et à 10 m au-dessus du fond. Les données de température et de salinité ont également été collectées à chaque instant à l'aide d'un CTD.\n\nÀ partir des échantillons d'ADNe, la communauté de poissons a été cartographiée à l'aide d'un métabarcoding ciblant une petite région du gène de l'ARNr 12S mitochondrial à l'aide du jeu d'amorces universelles miFISH-U. Des bibliothèques de séquençage indépendantes ont été préparées pour chacun des quatre ensembles de données saisonniers, et chacun a été séquencé à l'aide de la chimie Illumina MiSeq V3 au Hakai Genome Lab sur l'île Quadra, en Colombie-Britannique.\n\nLes profils d'ADNe spatiotemporels ont confirmé la présence d'eulakane (Thaleichthys pacificus) en voie de disparition dans trois fjords et ont identifié les habitats des fjords profonds comme des priorités de conservation pour les espèces mésopélagiques, notamment les élasmobranches sensibles et les migrateurs verticaux quotidiens tels que la lampfish nordique (Stenobrachius leucopsarus). Les pics d'ADNe de plusieurs CPS en hiver et au printemps correspondaient aux périodes de frai documentées, soulignant l'importance des fjords Les morues comme habitat de frai saisonnier. Cette approche transférable à l'échelle mondiale peut compléter les initiatives locales d'intendance et les efforts de surveillance à long terme, contribuant ainsi à une conception et à une gestion plus efficaces des aires marines de conservation."},"title_translated":{"en":"Putting fjord biodiversity on the map for British Columbia conservation planning","fr":"Mettre la biodiversité des fjords sur la carte pour la planification de la conservation en Colombie-Britannique"},"xml_location_url":"https://metadata-generator-proxy.server.hakai.app/metadata/hakai/putting_fjord_biodiversity_on__b4844.xml","metadata-language":"en","metadata_modified":"2026-10-02T23:11:00.114690","frequency-of-update":"asNeeded","dataset-reference-date":[{"type":"creation","value":"2023-01-31"},{"type":"publication","value":"2026-05-17"}],"cited-responsible-party":[{"role":["author","custodian","distributor","originator","owner","pointOfContact","processor","publisher","editor","contributor"],"individual-name":"Jacquemot, Loïc","organisation-name":"University of British 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