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The HPLC solvent protocol was based on gradient dilution with two solvent mixtures (Zapata et al. 2000): a methanol, acetonitrile, and aqueous pyridine (50:25:25 v:v:v) solution; and a methanol, acetonitrile, and acetone (20:60:20 v:v:v) solution. The flow rate was 1mL/ min, and the equilibration time was 7min.","fr":"Prélèvement d'échantillons : L'échantillonnage faisait partie de l'étude CASES (Canadian Arctic Shelf Exchange Study) dans le cadre de laquelle le NGCC Amundsen a été gelé dans la baie de Franklin, sur la côte de la mer de Beaufort (station FB/200) en hiver. Les communautés de plancton microbien des couches supérieures de la couche mixte ont été échantillonnées à 5 m sous la surface de l'eau à l'aide du système de rosette CTD du navire équipé de 12 L Niskins en eau libre. Pendant la période où le navire a été gelé dans la baie de Franklin, de décembre 2003 au début de juin 2004, des échantillons ont été prélevés à l'aide d'une bouteille Niskin de 5 L à 3 m sous le fond de la glace à travers un trou de 300 mm qui avait été foré à 500 m en amont du navire. Analyse des pigments par HPLC : des échantillons d'eau d'un à deux litres ont été filtrés sur des filtres Whatman GF/F et conservés congelés à -80 °C jusqu'à l'analyse. Les pigments de phytoplancton présents sur les GF/F ont été extraits dans 3 ml de MeOH à 95 % et 100 µL des extraits ont été injectés dans une HPLC Varian ProStar équipée d'une colonne Symmetry C8. Les pics HPLC ont été détectés par spectroscopie à matrice de diodes (350-750 nm) et des chromatogrammes d'absorbance ont été obtenus à 440 (pour les chls) et 450 nm (pour les caroténoïdes). Les chlorophylles ont également été détectées par fluorescence (excitation : 440 nm ; émission : 650 nm). Le protocole de solvant HPLC était basé sur une dilution en gradient avec deux mélanges de solvants (Zapata et al. 2000) : une solution de méthanol, d'acétonitrile et de pyridine aqueuse (50:25:25 v:v:v) ; et une solution de méthanol, d'acétonitrile et d'acétone (20:60:20 v:v). Le débit était de 1 mL/ min et le temps d'équilibrage était de 7 min."},"title_translated":{"en":"HPLC Pigment Analysis of the Phytoplankton Community in Franklin Bay","fr":"Analyse pigmentaire par HPLC de la communauté phytoplanctonique de Franklin Bay"},"xml_location_url":"https://waf.forms.cioos.ca/metadata/amundsen/center-for-northern-studies/ccin-226.xml","metadata-language":"en","metadata_modified":"2026-09-29T21:15:20.001394","frequency-of-update":"asNeeded","dataset-reference-date":[{"type":"creation","value":"2003-10-19"},{"type":"publication","value":"2016-01-14"},{"type":"revision","value":"2016-01-14"}],"cited-responsible-party":[{"role":"principalInvestigator","individual-name":"Vincent, Warwick","organisation-name":"","contact-info_email":"","contact-info_online-resource":""},{"role":"originator","individual-name":"Martineau, 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xlink:href=\"https://cioos.ca/translation_method\" xlink:role=\"translation\" xlink:title=\"text translated using the Amazon translate service / texte traduit à l&#39;aide du service de traduction Amazon\">\n                <lan:LocalisedCharacterString locale=\"#fr\">Analyse pigmentaire par HPLC de la communauté phytoplanctonique de Franklin Bay</lan:LocalisedCharacterString>\n              </lan:textGroup>\n            </lan:PT_FreeText>\n          </cit:title>\n          <cit:date>\n            <cit:CI_Date>\n              <cit:date>\n                <gco:Date>2003-10-19</gco:Date>\n              </cit:date>\n              <cit:dateType>\n                <cit:CI_DateTypeCode codeList=\"http://standards.iso.org/iso/19115/resources/Codelists/cat/codelists.xml#CI_DateTypeCode\" codeListValue=\"creation\">creation</cit:CI_DateTypeCode>\n              </cit:dateType>\n            </cit:CI_Date>\n          </cit:date>\n          <cit:date>\n            <cit:CI_Date>\n              <cit:date>\n                <gco:Date>2016-01-14</gco:Date>\n              </cit:date>\n              <cit:dateType>\n                <cit:CI_DateTypeCode codeList=\"http://standards.iso.org/iso/19115/resources/Codelists/cat/codelists.xml#CI_DateTypeCode\" codeListValue=\"publication\">publication</cit:CI_DateTypeCode>\n              </cit:dateType>\n            </cit:CI_Date>\n          </cit:date>\n          <cit:date>\n            <cit:CI_Date>\n              <cit:date>\n                <gco:Date>2016-01-14</gco:Date>\n              </cit:date>\n              <cit:dateType>\n                <cit:CI_DateTypeCode codeList=\"http://standards.iso.org/iso/19115/resources/Codelists/cat/codelists.xml#CI_DateTypeCode\" codeListValue=\"revision\">revision</cit:CI_DateTypeCode>\n              </cit:dateType>\n            </cit:CI_Date>\n          </cit:date>\n          <cit:edition>\n            <gco:CharacterString>1.0</gco:CharacterString>\n          </cit:edition>\n          <cit:citedResponsibleParty>\n            <cit:CI_Responsibility>\n              <cit:role>\n                <cit:CI_RoleCode codeList=\"https://standards.iso.org/iso/19115/resources/Codelists/cat/codelists.xml#CI_RoleCode\" codeListValue=\"principalInvestigator\"/>\n              </cit:role>\n              <cit:party>\n                <cit:CI_Individual>\n                  <cit:name>\n                    <gco:CharacterString>Vincent, Warwick</gco:CharacterString>\n                  </cit:name>\n                  <cit:contactInfo>\n                    <cit:CI_Contact></cit:CI_Contact>\n                  </cit:contactInfo>\n                  <cit:positionName>\n                    <gco:CharacterString></gco:CharacterString>\n                  </cit:positionName>\n                </cit:CI_Individual>\n              </cit:party>\n            </cit:CI_Responsibility>\n          </cit:citedResponsibleParty>\n          <cit:citedResponsibleParty>\n            <cit:CI_Responsibility>\n              <cit:role>\n                <cit:CI_RoleCode codeList=\"https://standards.iso.org/iso/19115/resources/Codelists/cat/codelists.xml#CI_RoleCode\" codeListValue=\"originator\"/>\n              </cit:role>\n              <cit:party>\n                <cit:CI_Individual>\n                  <cit:name>\n                    <gco:CharacterString>Martineau, Marie-Josée</gco:CharacterString>\n                  </cit:name>\n                  <cit:contactInfo>\n                    <cit:CI_Contact></cit:CI_Contact>\n                  </cit:contactInfo>\n                  <cit:positionName>\n                    <gco:CharacterString></gco:CharacterString>\n                  </cit:positionName>\n                </cit:CI_Individual>\n              </cit:party>\n            </cit:CI_Responsibility>\n          </cit:citedResponsibleParty>\n        </cit:CI_Citation>\n      </mri:citation>\n      <mri:abstract xsi:type=\"lan:PT_FreeText_PropertyType\">\n        <gco:CharacterString>Sample collection: The sampling was part of the Canadian Arctic Shelf Exchange Study (CASES) in which CCGS Amundsen was frozen in Franklin Bay in the coastal Beaufort Sea (Station FB/200) during winter. Upper mixed-layer microbial plankton communities were sampled 5m below the water surface using the ship CTD rosette system equipped with 12L Niskins during openwater conditions. During the time that the ship was frozen in Franklin Bay from December 2003 to early June 2004, samples were taken using a 5L Niskin bottle from 3m below the bottom ice through a 300mm hole that had been drilled 500m upstream of the ship. HPLC pigment analysis: One to two liter samples of water were filtered onto Whatman GF/F filters and stored frozen at -80C until analysis. Phytoplankton pigments on the GF/Fs were extracted in 3mL of 95 percent MeOH and 100 uL of the extracts was injected into a Varian ProStar HPLC equipped with a Symmetry C8 column. The HPLC peaks were detected by diode-array spectroscopy (350-750nm) and absorbance chromatograms were obtained at 440 (for chls) and 450nm (for carotenoids). Chlorophylls were also detected by fluorescence (excitation: 440nm; emission: 650nm). The HPLC solvent protocol was based on gradient dilution with two solvent mixtures (Zapata et al. 2000): a methanol, acetonitrile, and aqueous pyridine (50:25:25 v:v:v) solution; and a methanol, acetonitrile, and acetone (20:60:20 v:v:v) solution. The flow rate was 1mL/ min, and the equilibration time was 7min.</gco:CharacterString>\n        <lan:PT_FreeText>\n          <lan:textGroup xlink:href=\"https://cioos.ca/translation_method\" xlink:role=\"translation\" xlink:title=\"text translated using the Amazon translate service / texte traduit à l&#39;aide du service de traduction Amazon\">\n            <lan:LocalisedCharacterString locale=\"#fr\">Prélèvement d&#39;échantillons : L&#39;échantillonnage faisait partie de l&#39;étude CASES (Canadian Arctic Shelf Exchange Study) dans le cadre de laquelle le NGCC Amundsen a été gelé dans la baie de Franklin, sur la côte de la mer de Beaufort (station FB/200) en hiver. Les communautés de plancton microbien des couches supérieures de la couche mixte ont été échantillonnées à 5 m sous la surface de l&#39;eau à l&#39;aide du système de rosette CTD du navire équipé de 12 L Niskins en eau libre. Pendant la période où le navire a été gelé dans la baie de Franklin, de décembre 2003 au début de juin 2004, des échantillons ont été prélevés à l&#39;aide d&#39;une bouteille Niskin de 5 L à 3 m sous le fond de la glace à travers un trou de 300 mm qui avait été foré à 500 m en amont du navire. Analyse des pigments par HPLC : des échantillons d&#39;eau d&#39;un à deux litres ont été filtrés sur des filtres Whatman GF/F et conservés congelés à -80 °C jusqu&#39;à l&#39;analyse. Les pigments de phytoplancton présents sur les GF/F ont été extraits dans 3 ml de MeOH à 95 % et 100 µL des extraits ont été injectés dans une HPLC Varian ProStar équipée d&#39;une colonne Symmetry C8. Les pics HPLC ont été détectés par spectroscopie à matrice de diodes (350-750 nm) et des chromatogrammes d&#39;absorbance ont été obtenus à 440 (pour les chls) et 450 nm (pour les caroténoïdes). Les chlorophylles ont également été détectées par fluorescence (excitation : 440 nm ; émission : 650 nm). Le protocole de solvant HPLC était basé sur une dilution en gradient avec deux mélanges de solvants (Zapata et al. 2000) : une solution de méthanol, d&#39;acétonitrile et de pyridine aqueuse (50:25:25 v:v:v) ; et une solution de méthanol, d&#39;acétonitrile et d&#39;acétone (20:60:20 v:v). 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