EDNA data for Crown of Thorns Seastar (Acanthaster cf. solaris) - CCIP-D-03
AIMS developed an eDNA based methods to detect and quantify CoTS. The data included here include field data from initial method development as part of AIMS appropriation, and later testing through several CoTS Control Innovation Program (CCIP) projects. Data consist of filtered water samples, in most cases collected with 12 replicates at three sites per reef.
Data Dictionary:
Field name: Explanation/units
Date: Julian Date
Year: Collection Year
Reef: GBR Reef Name
Site_name: Site name
Site_No: Site number
Lat: Latitude in decimal degrees
Long: Longitude in decimal degrees
Project: Project Code
Reef_rep: replicate number on a reef basis
Site_rep: replicate number on a site
PCR1_binary: First technical PCR replicate: positive or negative (1 or 0)
PCR2_binary: First technical PCR replicate: positive or negative (1 or 0)
LOD_sample_positive: Limit of Detection based: 1 if either of the technical replicates are positive
PCR1_conc: CoTS eDNA copy number per L water filtered, first technical replicate
PCR2_conc: CoTS eDNA copy number per L water filtered, second technical replicate
Conc_mean: CoTS eDNA copy number per L water filtered, averaged over both replicates
Data and resources
-
Qiacube DNA extraction of eDNA filter samples stored in 540ul ATL PDFsize: 166 KBPDF Open
-
Uthicke, S., Gomez Cabrera, M., Patel, F., Lawerence, E., Tabor, B., Foster, S., & Doyle, J. (2025). Operationalising and implementing crown-of-thorns starfish…PDF Open
-
CoTS eDNA sampling with standard filter housings PDF size: 753 KBPDF Open
-
Uthicke, S., Doyle, J. R., Gomez Cabrera, M., Patel, F., McLatchie, M. J., Doll, P. C., Chandler, J. F., & Pratchett, M. S. (2024). eDNA monitoring detects new…HTML Open
-
Associated metadata record for Uthicke et al (2022)HTML Open
-
Uthicke, S., Robson, B., Doyle, J. R., Logan, M., Pratchett, M. S., & Lamare, M. (2022). Developing an effective marine eDNA monitoring: EDNA detection at…HTML Open
-
BioRad ddPCR AutoDG workflow PDF size: 400 KBPDF Open
-
audio/basic Open
-
audio/basic Open
-
audio/basic Open
-
audio/basic Open
-
License GraphicPNG Open
-
HTML Open
Additional information
| Date (revision) | 2026-10-24 |
|---|---|
| Status | completed |
| Update frequency | asNeeded |
| Metadata language | en |
| License | Creative Commons Attribution 4.0 |
| Source metadata | ISO 19115 XML |
| Last updated | 2026-11-05 |
| Created | 2026-11-05 |
| API | package_show |